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大鼠肺泡巨噬細(xì)胞NR8383

屬	大鼠
別稱	NR-8383; NR 8383; NR8383.1; AgC11x3A; Normal Rat, August 3, 1983
組織來源	肺；肺泡
疾病	自發(fā)永生細(xì)胞系
傳代比例/細(xì)胞消化	1:2傳代,懸浮部分離心收集(1000RPM,5分鐘) ，貼壁部分消化1-2分鐘
培養(yǎng)基配置	Ham's F-12K培養(yǎng)基; 20%胎牛血清；1%雙抗
簡介	NR8383細(xì)胞來源于肺灌洗時的正常大鼠肺泡巨噬細(xì)胞，NR8383細(xì)胞在Gerbil肺細(xì)胞連續(xù)培養(yǎng)液存在下培養(yǎng)了8-9個月。隨后，NR8383細(xì)胞不再需要外源生長因子。通過有限稀釋法，從單個細(xì)胞克隆并亞克隆NR8383細(xì)胞，并3次用軟瓊脂亞克隆。NR8383細(xì)胞表現(xiàn)出巨噬細(xì)胞的特性：吞噬酵母多糖和銅綠、非特異性脂酶活性、Fc受體、氧化降解；分泌IL-1、TNF-β和IL-6 ，可重復(fù)地響應(yīng)外源生長因子。NR8383細(xì)胞響應(yīng) ，分泌TNF-β前體。 NR8383細(xì)胞在刺激下，TNF-β mRNA表達(dá)也上升。NR8383細(xì)胞對內(nèi)毒素敏感，1-10ng/ml的LPS水平抑制增生達(dá)50%。即使達(dá)到0.001mg/ml的水平，LPS抑制還是無毒且在后續(xù)過程中可逆的。NR8383細(xì)胞提供了高響應(yīng)的肺泡巨噬細(xì)胞的均一來源，可以用于體外研究巨響細(xì)胞相關(guān)活性
形態(tài)	巨噬細(xì)胞樣
生長特征	貼壁，懸浮混合生長
倍增時間	每周 2 至 3 次
培養(yǎng)條件	氣相：空氣，95% ；二氧化碳，5%。溫度：37攝氏度，培養(yǎng)箱濕度為70%-80%。
凍存條件	凍存液：90%FBS ，DMSO 10%, 或使用非程序凍存液：貨號JY-H040
保藏機(jī)構(gòu)	ATCC; CRL-2192
備注	該細(xì)胞為半懸浮和半貼壁細(xì)胞，懸浮細(xì)胞離心收集，貼壁細(xì)胞消化處理
產(chǎn)品使用	于科學(xué)研究，不可作為動物或人類疾病的治療產(chǎn)品使用。

cowpea-wheat crop sequence. Appl. Soil Ecol. 157, 103764
Galvez, A., Sinicco, T., Cayuela, g.L., gingorance, g.D., Fornasier, F., gondini, C.,
atientswithAISofditerentseverities were compared , andthecorrelationbetween serum OccludinlevelsandNIHSSscoresin p atientswith AIS was
as a p redictorofAISoccurrence , hemorrha g ictransformation and p oor p ro g nosis .
Keywords:Acuteischemicstroke ; Occludin; Bloodbrainbarrier
Both resistance training (RT) and long-duration, high-intensity stretching induce muscular adaptations; however, it is unknown whether the modalities are complementary or redundant, particularly in well-trained individuals. A case-study was conducted on a competitive bodybuilder implementing long-duration, high-intensity stretching of the plantar flexors (60?min 6x/week for 12?weeks) in conjunction with their habitual RT. Ultrasound muscle architecture (muscle thickness [MT], fascicle length [FL], and pennation angle [PA]) measurements were collected at multiple sites at four weekly baseline sessions, six (mid) and 12 (post1) weeks following the commencement of the intervention, and a week after the intervention (post2) while isometric strength and range of motion (RoM) were obtained once at baseline, mid, post1, and post2. 2SD bａnd plots were constructed to determine meaningful changes in MT, FL, and PA from the four baseline measures while percentage and absolute change across each timepoint were calculated for all variables. From baseline to post 1, RoM, strength, and MT increased 25.9%, 11.4%, and 7.4%-23.4%, respectively, while four MT and two PA sites exceeded the threshold for meaningful change. The combined stretching and RT protocols resulted in flexibility, strength, and MT adaptations; however, findings should be generalized with caution given the case-study nature of our investigation.