目錄:北京北納創(chuàng)聯(lián)生物技術(shù)研究院>>ATCC>>ATCC細(xì)胞>> ATCCPRA-302WA1 ATCC細(xì)胞代理
公司介紹
北京北納創(chuàng)聯(lián)生物技術(shù)研究院是一家專業(yè)從事標(biāo)準(zhǔn)物質(zhì)、計(jì)量分析儀器和精細(xì)化工產(chǎn)品研發(fā)及銷售的科研單位。是國內(nèi)標(biāo)準(zhǔn)物質(zhì)提供商之一，代理銷售國內(nèi)外數(shù)萬種標(biāo)準(zhǔn)物質(zhì)、微生物、菌種、細(xì)胞類等產(chǎn)品。
北京標(biāo)準(zhǔn)物質(zhì)網(wǎng)隸屬于北京北納創(chuàng)聯(lián)生物技術(shù)研究院，具備強(qiáng)大的分析測試、技術(shù)開發(fā)、*、物流管理和售后服務(wù)能力，并與國內(nèi)外眾多檢測機(jī)構(gòu)、科研院校和工廠企業(yè)有著良好和穩(wěn)定的合作關(guān)系，具有較強(qiáng)的性和影響力。
北納生物也隸屬于北京北納創(chuàng)聯(lián)生物技術(shù)研究院，實(shí)驗(yàn)基地總占地面積16畝，并設(shè)有細(xì)胞庫、微生物菌種保藏庫，細(xì)胞培養(yǎng)、菌種培養(yǎng)等實(shí)驗(yàn)室。擁有細(xì)胞和菌株2萬余株，并設(shè)有生物資源信息庫，收錄信息達(dá)20余萬株。致力于提供實(shí)驗(yàn)室綜合解決方案，為廣大客戶服務(wù)。
經(jīng)營范圍：
國家標(biāo)準(zhǔn)物質(zhì)、國內(nèi)外標(biāo)準(zhǔn)品、對照品、實(shí)驗(yàn)耗材、試劑、菌株、細(xì)胞、ATCC、培養(yǎng)基、血清、冶金標(biāo)樣、標(biāo)準(zhǔn)氣體、試劑盒等，歡迎查詢！
ATCCPRA-302 WA1 ATCC細(xì)胞代理
| 基本信息 | |
| 資源編號 | ATCCPRA-302 |
| 資源名稱 | WA1 |
| 種屬 | Babesia duncani |
| 分離基物 | Human blood, Washington State, 1991 |
| 提供形式 | frozen |
| 安全等級 | 2 |
| 模式菌株 | no |
| 應(yīng)用領(lǐng)域 | Vector borne research |
| 培養(yǎng)方法 | |
| 生長條件 | Temperature: 25°C Culture System: in vivo c*tion, hamster |
| 存儲(chǔ)條件 | Frozen Cultures:-70°C for 1 week; liquid N2 vapor for long term storageFreeze-dried Cultures:2-8°CLive Cultures:See Protocols section for handling information;Reagents Alsever's Solution NaCl, 4.2 g Na3citrate·2H2O, 8.0 g Glucose, 20.5 g Glass distilled H2O to 1.0 L *Dissolve components in glass distilled H2O, adjust the pH to 6.1 with 10% (w/v) citric acid and filter sterilize. The solution can be obtained from Sigma-Aldrich (cat# A3551). Harvest and Preservation Prepare a 30% (v/v) sterile glycerol solution in Alsever's solution. Draw approximately 0.5 mL of anticoagulant solution (Yaeger's or heparin, etc.) into a syringe and move it back and forth over the length of the syringe, several times. Remove all air bubbles. Draw blood by cardiac puncture into the syringe from a host animal that has reached or is near peak parasitemia. If clotting occurs during extraction of blood, insufficient heparin was used. Mix the heparinized blood with the 30% glycerol solution in a 2:1 ratio. If any clotting has occurred do not use. After mixing, the final concentration of cryoprotectant solution will be 10% (v/v). The mixture should be placed in a 4°C ice bath. The time from the mixing of the cell preparation and glycerol stock solution before the freezing process is begun should be no less than 15 min and no longer than 30 min. Dispense in 0.5 mL aliquots into 1.0 mL to 2.0 mL sterile plastic screw-capped cryules (special plastic vials for cryopreservation). Filled ampules should be placed in a 4°C ice bath. Do not immerse ampules to the level of the vial cap. Plunge ampules from 4°C into liquid nitrogen. The frozen preparations may be stored in a mechanical freezer until needed, however storage in either the vapor or liquid phase of a nitrogen refrigerator is recommended for the longest viability. To thaw a frozen ampule, place in a 35°C water bath, until thawed (2 to 3 min). Immerse the ampule just sufficient to cover the frozen material. Do not agitate the ampule. Immediately after thawing, aseptically remove the contents of the ampule with a syringe and inoculate an uninfected hamster. Follow the protocol for maintenance in vivo. The course of infection may be longer or shorter than usual depending on percent recovery of the parasite from the frozen state. |
| 詳細(xì)說明 | |
| 描述 | Strain has been passaged in vivo in jirds several times |
| Year of Origin | 1991 |
ATCCPRA-302 WA1 ATCC細(xì)胞代理
溫馨提示：
本店商品種類眾多，因鋪位有限商品未全部上架，如果沒有找到您想要的商品可以咨詢客服，我們會(huì)耐心解答，祝您購物愉快！
(空格分隔,最多3個(gè),單個(gè)標(biāo)簽最多10個(gè)字符)
員.png)
8
化工儀器網(wǎng)