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Iodoacetamide（胺鹽）

說明：蛋白組學(xué)中組氨酸和半胱氨酸的烷基化試劑，用于多肽測序以及酶的不可逆抑制。

別名：碘乙酰胺；2-碘乙酰胺；2-Iodoacetamide；Monoiodoacetamide

分子式：C 2 H 4 INO 分子量：184.96

C A S ＃：144-48-9

外觀：白色黃色結(jié)晶

特性：純度：≥99% (TLC)

熔點(diǎn)：92-95 ℃

溶解性：溶于水，參考濃度100mg/ml。

R：25-42/43 S：22-36/37-45

儲(chǔ)存條件：2-8℃

Iodoacetamide（胺鹽）結(jié)構(gòu)式：

IODOACETAMIDE
Product Number I6125 AND I1149
Storage Temperature 2-8 °C
CAS #: 144-48-9
Synonyms: IAN

1 ; a-Iodoacetamide; Monoiodoacetamide; 2-Iodoacetamide; Surauto; USAF D-1

2 Product Description Appearance: powder.

3 Melting Point: 93°C-96°C 2 Molecular Formula: C 2 H 4 INO Formula Weight: 185.0
Some enzymes IAN reportedly inhibits (to varyingextents) are: Acetoacetyl-CoA Thiolase

5 ; Adenylate Kinase

6 ; Alcohol Dehydrogenase (human liver)

7 ;Aldehyde Dehydrogenase (0.1 mM, 40%) 

8; Alkaline
Phosphatase (calf intestinal)

9,10 ; d-Aminolevulinate Dehydratase

11 ; ?-Amylase (soybean)

12 ; L-Arginine 2- Monoxygenase

13 ; Benzaldehyde Dehydrogenase 1 (0.5-10 mM)

14 ; Calcium-Activated Protease (100 μM,100%)

15 ; Carbonic Anhydrase B(human)

16 ; CathepsinB (1 mM in the presence of 0.04 M cysteine)

17 ;Cyclohexanone Oxygenase (5 mM, 67%)

18 ; DolicholEsterase

19; Formylmethionine Deformylase (10 mM,80%)

20 Galactose Oxidase (0.1 mM, 22%)

21 ; Gamma-Glutamylcyclotransferase(1mM, 14%)

22 ; GlutathioneThiol Esterase

23 Glyceraldehyde-3-Phosphate:NADPOxidoreductase (0.4 mM, 44%)

24 ; Heme Oxygenase (5mM, 39%) 

25 ?-Hydroxy-?-Methylglutaryl Coenzyme A Reductase

26 ; 3-Ketoacyl-CoA-Thiolase (0.1 M, 84%)

27 ;L-Lysine Decarboxylase (1 mM)

28 ; L-?-Lysine Mutase

29 ;Malate Dehydrogenase (porcine)

30 ; NADPH-Linked a,?-Ketoalkene Double Bond Reductase (1 mM, 37%)

31 6-Phosphogluconate Dehydrogenase (10 mM, 80%)

32 ;Polyamine Oxidase

33 ; Prolidase

34 D-ProlineReductase

35 ; Prostaglandin Endoperoxide E Isomerase(1 mM, 47%)

36 Protease La (E.coli)

37 ; PyroglutamylPeptidase I (=Pyrrolidonecarboxylyl Peptidase)

38 ;Serine-Glyoxylate Aminotransferase (1 mM, 60%)
39 ;soluble metalloendopeptidase from rat brain (1 mM,14%)

40 ; Thioesterase component from fatty acid synthetase multienzyme complex (I 50 =100 μM)

41 ;Thioltransferase (human red blood cells)

42 ;Transglutaminase (in the presence of Ca 2+ )

43 ;Tryptophan Oxidative Decarboxylase (1 mM, 80%)

44 ;Tryptophan Synthetase

45 .IAN may also inhibit bromelain, cathepsin C, clostripainand carboxypeptidase P since these enzymes areinhibited by sulfhydryl agents such as iodoacetic acid. 1
IAN (irreversibly) inhibited the dephosphorylation of both epidermal growth factor receptor (EGFR) andplaet-derived growth factor receptor (PDGFR) presumably by alkylation at the active center of one orseveral protein tyrosine phosphatases in cell lines.

46IAN is an irreversible inhibitor of many cysteine proteases. However, it is not highly specific for the active site cysteine residue of proteases and can inhibit
many enzymes. In the alkylation reaction, IAN reactswith histidine (such as in RNase 47 ), methionine andsulfhydryl groups of many proteins. IAN can react with
low molecular weight thiol compounds such asmercaptoethanol and glutathione. 1 The reaction of IANwith glutathione was utilized in a titration method for thedetermination of sulfhydryl groups in differentcompounds. 48 The alkylation reaction forms stableprotein derivatives which will remain intact during further study of the protein.

49-52  Prod. No. I6125 is reagent grade. Product no.
I1149 has been additionally tested for trace metal
content.

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